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KMID : 0043320100330081169
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Archives of Pharmacal Research
2010 Volume.33 No. 8 p.1169 ~ p.1173
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Kaempferol glycosides and cardenolide glycosides, cytotoxic constituents from the seeds of Draba nemorosa (Brassicaceae)
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Moon Surk-Sik
Rahman Aziz Abdur
Manir Maniruzzaman
Ahamed V. S. Jamal
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Abstract
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Bioassay-directed fractionation of a methanolic extract from the seeds of Draba nemorosa (Brassicaceae) led to isolation of a new flavonol glycoside, drabanemoroside (5, kaempferol 3-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranose) along with four known flavonoid derivatives (1?4), four cardenolide glycosides (6?9). Kaempferol glycosides 2 and 5 showed strong cytotoxicity against human small lung cancer cell line A549 and melanoma SK-Mel-2 with an IC50 of 0.5 μg/mL and 1.9 μg/mL, respectively. Cardenolide glycosides 6?9 showed potent cytotoxicity (A549) in the range of 0.01?0.032 μg/mL. Their structures were characterized based on spectroscopic data (2D NMR, HRTOFMS, IR, and UV) and comparison of literature values. The carbohydrate units were also confirmed by comparing the hydrolysate of 5 with authentic monosaccharides.
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KEYWORD
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Draba nemorosa, Drabanemoroside, Kaempferol rhamnopyranosyl-arbinopyranoside, Cytotoxicity, Cardenolides
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